FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200376

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200376
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9094326
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN173450.19072331473492374No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG92740.10197567142413852No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT562000.074.121421
AGCAGTG629150.066.059812
CAGTGGT815700.050.790954
GCAGTGG842050.049.3634153
AGTGGTA855250.048.56385
TGGTATC890750.046.486547
GTGGTAT895250.046.2995646
GGTATCA910500.045.5177768
GTATCAA924200.044.8033949
CAACGCA911050.026.04442612-13
ATCAACG914500.025.95092210-11
ACGCAGA953650.024.95696314-15
AGAGTAC947550.024.80917518-19
GCAGAGT958800.024.71521616-17
TACATGG939200.024.57627322-23
AGTACAT950200.024.52760320-21
TCAACGC905050.022.96812212-13
CATGGGA381600.022.90291224-25
CATGGGG377250.022.5248824-25
CGCAGAG929000.022.4730416-17
Produced by FastQC (version 0.11.5)