FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200380

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200380
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9112963
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN124300.13639910531843485No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG91480.10038447429228012No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT579900.074.2827151
AGCAGTG651600.065.934242
CAGTGGT848650.050.607034
GCAGTGG875900.049.174053
AGTGGTA893850.048.08285
TGGTATC927900.046.2087337
GTGGTAT931600.046.1207246
GGTATCA946650.045.255098
GTATCAA960150.044.6035429
CAACGCA941800.026.19486412-13
ATCAACG945250.026.08201610-11
ACGCAGA986100.025.08326714-15
AGAGTAC978800.024.96177118-19
GCAGAGT991100.024.84157216-17
AGTACAT977800.024.77201820-21
TACATGG965300.024.76843822-23
CATGGGA394250.022.998824-25
TCAACGC937000.022.89364612-13
CATGGGG387500.022.56588624-25
CGCAGAG961050.022.44780716-17
Produced by FastQC (version 0.11.5)