FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200382

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200382
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9106961
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN173970.19102969695379174No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT568600.073.978711
AGCAGTG638150.065.861892
CAGTGGT822800.050.9986764
GCAGTGG851450.049.476163
AGTGGTA867250.048.5036965
TGGTATC901450.046.601397
GTGGTAT904050.046.4668586
GGTATCA923150.045.5949138
GTATCAA934900.044.9728669
CAACGCA919550.026.15475312-13
ATCAACG921900.026.11062210-11
ACGCAGA959500.025.07918514-15
ACGACGC4250.024.9914345
AGAGTAC955200.024.91769218-19
GCAGAGT963900.024.8953316-17
TACATGG943450.024.75292422-23
AGTACAT958050.024.60436420-21
CATGGGA383100.023.19067224-25
TCAACGC913800.023.00848612-13
CATGGGG373300.022.62687124-25
Produced by FastQC (version 0.11.5)