FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200386

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200386
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9860736
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT629400.074.4510961
AGCAGTG709100.065.9752662
CAGTGGT916550.050.9090424
GCAGTGG946850.049.3447843
AGTGGTA959750.048.5984235
TGGTATC1002450.046.38447
GTGGTAT1007750.046.2233966
GGTATCA1025750.045.4297378
GTATCAA1043600.044.752919
CAACGCA1026100.026.20469912-13
ATCAACG1028100.026.18998110-11
ACGCAGA1070050.025.19049314-15
AGAGTAC1062750.024.94698318-19
GCAGAGT1076650.024.85025216-17
TACATGG1050050.024.78233522-23
AGTACAT1063950.024.7616720-21
CATGGGA425200.023.50451924-25
TCAACGC1020400.022.94470412-13
CATGGGG415900.022.8853324-25
CGCAGAG1041650.022.52246916-17
Produced by FastQC (version 0.11.5)