FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200388

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200388
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9888203
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT627400.074.119611
AGCAGTG702900.066.02762
CAGTGGT917500.050.4365844
GCAGTGG945750.049.0187033
AGTGGTA961000.048.2199635
TGGTATC1001500.046.1398937
GTGGTAT1003250.046.13086
GGTATCA1023000.045.2291348
GTATCAA1042950.044.4556739
CAACGCA1022750.026.02128212-13
ATCAACG1025800.025.96251310-11
ACGACGC3350.025.315985
ACGCAGA1068750.024.95607414-15
AGAGTAC1061650.024.8286118-19
GCAGAGT1069950.024.77565216-17
TACATGG1047800.024.72599822-23
AGTACAT1062400.024.63772420-21
CATGGGA425650.023.30811324-25
TCAACGC1016300.022.79730212-13
CATGGGG418000.022.3872824-25
Produced by FastQC (version 0.11.5)