FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200391

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200391
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8022021
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGACTT34750.018.3931851
AAGGCGA8900.018.2912067
GCATGAC24900.017.890667
ACGGCCT25050.017.73972910
CCCGGAA16800.017.6803191
CGTGAGC20100.017.6222044
TTGGGCG12050.017.5287041
GCGCATG17150.017.5105175
GAGACCC23000.017.4582481
AGAGAGT18100.017.4411058
AGGGCGT8350.017.389643
GTAGCGC19300.017.3825388
AACGGCC25800.017.3093099
TCAACGG25700.017.2909537
CGGGAAG12300.017.2605783
GGGGTGA8100.017.2472215
CGCATGG26300.017.1499841
CGCGCTA13250.017.10112610
ATACCCG22450.017.1006342
ACGGAAG15650.017.0815621
Produced by FastQC (version 0.11.5)