FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200392

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200392
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8022021
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG94050.11723978284275247No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT502600.074.183421
AGCAGTG566000.065.780152
CAGTGGT742550.049.9364554
GCAGTGG770000.048.3284233
AGTGGTA775950.047.873015
TGGTATC815050.045.4807437
GTGGTAT815150.045.4783746
GGTATCA831600.044.6327938
GTATCAA843650.043.944979
CAACGCA829500.025.70625112-13
ATCAACG832000.025.62960810-11
ACGCAGA864500.024.72155414-15
AGAGTAC860150.024.49651518-19
GCAGAGT870550.024.4444116-17
TACATGG847500.024.33773622-23
AGTACAT860500.024.21316520-21
CATGGGA341750.022.63643324-25
TCAACGC824450.022.60535212-13
CATGGGG339100.022.40595224-25
CGCAGAG844800.022.1846416-17
Produced by FastQC (version 0.11.5)