FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200398

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200398
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8127566
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG86030.10584964797579004No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT516450.074.448081
AGCAGTG582950.065.954652
CAGTGGT757500.050.6669654
GCAGTGG782350.049.196683
AGTGGTA796400.048.334715
TGGTATC826250.046.466837
GTGGTAT830200.046.314976
GGTATCA842850.045.5695658
GTATCAA856000.044.7776039
CAACGCA839650.026.28561612-13
ATCAACG843900.026.16471510-11
AGAGTAC872250.025.07426818-19
ACGCAGA881700.025.0459914-15
GCAGAGT884400.024.93904316-17
TACATGG861000.024.88541222-23
AGTACAT872650.024.81829820-21
CATGGGG348950.023.04936824-25
TCAACGC837250.022.9610212-13
CATGGGA348550.022.85018224-25
CGCAGAG857450.022.5189816-17
Produced by FastQC (version 0.11.5)