FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200400

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200400
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9327017
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG96150.1030876216908364No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT588850.074.279431
AGCAGTG664900.065.726462
CAGTGGT861850.050.623264
GCAGTGG899600.048.5848273
AGTGGTA906150.048.1730465
GTGGTAT944100.046.2186746
TGGTATC943550.046.1874547
GGTATCA965850.045.159088
GTATCAA982050.044.4575239
CAACGCA962650.026.09169612-13
ATCAACG965400.026.04862810-11
ACGCAGA1006050.025.00109514-15
AGAGTAC995450.024.99456818-19
GCAGAGT1011650.024.74941816-17
TACATGG989350.024.66464422-23
AGTACAT1003150.024.5781620-21
CATGGGA395850.022.98520524-25
CATGGGG394200.022.83339924-25
TCAACGC957050.022.78323212-13
CGCAGAG978100.022.33426316-17
Produced by FastQC (version 0.11.5)