FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200402

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200402
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9526502
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT615500.073.6147461
AGCAGTG685150.065.9232252
CAGTGGT885450.050.921554
GCAGTGG914100.049.434473
AGTGGTA929400.048.603755
TGGTATC963000.046.700637
GTGGTAT969150.046.4885756
GGTATCA984850.045.725298
GTATCAA1001050.045.0050969
ACGACGC2950.031.605065
CAACGCA981350.026.4523412-13
ATCAACG986400.026.3210110-11
AGAGTAC1021850.025.1978718-19
ACGCAGA1033150.025.19116614-15
GCAGAGT1030250.025.14792416-17
AGTACAT1020650.024.9366520-21
TACATGG1008850.024.92302522-23
CATGGGA408550.023.4623524-25
TCAACGC976550.023.13850812-13
CATGGGG401050.022.68751524-25
Produced by FastQC (version 0.11.5)