FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200406

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200406
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9473595
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT604350.073.5494461
AGCAGTG677500.065.489732
CAGTGGT882550.050.15924
GCAGTGG900400.049.2754753
AGTGGTA920200.048.1931765
GTGGTAT964550.045.9357036
TGGTATC964550.045.870977
GGTATCA983100.045.068688
GTATCAA1001050.044.2913749
CAACGCA985750.025.95384212-13
ATCAACG988500.025.91585210-11
ACGACGC4850.025.3508385
ACGCAGA1026900.024.96550214-15
AGAGTAC1019600.024.84191318-19
GCAGAGT1027350.024.83360516-17
TACATGG1004000.024.69543522-23
AGTACAT1018450.024.64121820-21
CATGGGA401700.023.15390224-25
TCAACGC978750.022.69783812-13
CATGGGG407200.022.56974624-25
Produced by FastQC (version 0.11.5)