FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200410

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200410
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8648608
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT551850.073.576341
AGCAGTG614850.065.919892
CAGTGGT799550.050.5778124
GCAGTGG827400.049.0876243
AGTGGTA834800.048.4201165
TGGTATC873200.046.17997
GTGGTAT875600.046.0769926
GGTATCA889150.045.4070938
GTATCAA902850.044.6825949
ACGACGC4500.026.3664075
CAACGCA884650.026.3016912-13
ATCAACG889200.026.1708710-11
ACGCAGA926550.025.17300214-15
AGAGTAC917350.025.03358318-19
GCAGAGT930300.024.96605116-17
TACATGG905100.024.91967222-23
AGTACAT920050.024.77007920-21
CATGGGA372000.023.18039524-25
TCAACGC880750.022.99910712-13
CATGGGG356900.022.84498224-25
Produced by FastQC (version 0.11.5)