FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200412

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200412
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8653297
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT551250.073.91661
AGCAGTG619400.065.728372
CAGTGGT805200.050.5501334
GCAGTGG832150.048.965663
AGTGGTA839800.048.4694185
TGGTATC875900.046.33817
GTGGTAT879600.046.194876
GGTATCA895600.045.401158
GTATCAA909600.044.705479
ACGACGC3600.029.4448915
CAACGCA893750.026.2396812-13
ATCAACG895500.026.18485610-11
ACGCAGA933000.025.19568414-15
AGAGTAC924350.025.07071518-19
GCAGAGT937000.024.9391816-17
TACATGG913650.024.87822722-23
AGTACAT925100.024.79900720-21
CATGGGA374850.023.35953324-25
TCAACGC888700.022.9592212-13
CGCAGAG908850.022.51635716-17
Produced by FastQC (version 0.11.5)