FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200416

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200416
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9527517
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT622850.074.196231
AGCAGTG694300.066.346072
CAGTGGT903800.050.8730854
GCAGTGG936950.049.2354773
AGTGGTA948300.048.5408445
TGGTATC992250.046.2810487
GTGGTAT993550.046.2401736
GGTATCA1016100.045.27128
GTATCAA1033200.044.5235759
CAACGCA1015400.025.98298512-13
ATCAACG1018200.025.92511710-11
ACGACGC3700.025.2606855
ACGCAGA1057000.024.95487414-15
AGAGTAC1048300.024.84068318-19
GCAGAGT1061300.024.72267216-17
AGTACAT1047600.024.66392320-21
TACATGG1038250.024.6304822-23
CATGGGA421750.023.13965224-25
TCAACGC1009150.022.80880512-13
CATGGGG417600.022.39757324-25
Produced by FastQC (version 0.11.5)