FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200422

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200422
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9593664
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT620550.074.443491
AGCAGTG701600.065.789632
CAGTGGT910000.050.647934
GCAGTGG936150.049.2893333
AGTGGTA949450.048.625585
TGGTATC990500.046.589637
GTGGTAT992600.046.4480066
GGTATCA1012200.045.6463558
GTATCAA1029750.044.8353849
CAACGCA1011250.026.3445512-13
ATCAACG1013900.026.2975810-11
ACGCAGA1055700.025.25501614-15
AGAGTAC1047150.025.11877418-19
GCAGAGT1062550.024.95846216-17
AGTACAT1048300.024.89712120-21
TACATGG1036700.024.89557522-23
CATGGGA425850.023.3119224-25
CATGGGG406950.023.17251824-25
TCAACGC1006300.022.9983112-13
CGCAGAG1027700.022.54173716-17
Produced by FastQC (version 0.11.5)