FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200426

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200426
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7958788
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG83410.10480238950955847No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT513850.074.517861
AGCAGTG576950.066.35342
CAGTGGT751400.050.9381264
GCAGTGG772150.049.718963
AGTGGTA785100.048.8419155
TGGTATC817650.046.7229237
GTGGTAT822350.046.5319336
GGTATCA836750.045.6987578
GTATCAA847700.045.0084769
ACGACGC3500.029.138145
CAACGCA835500.026.1655912-13
ATCAACG837050.026.14120710-11
ACGCAGA872700.025.09282714-15
AGAGTAC867050.025.0364718-19
TACATGG855800.024.84793522-23
GCAGAGT882750.024.81763516-17
AGTACAT867200.024.81435420-21
CATGGGA346400.023.28357524-25
CATGGGG341150.023.02514624-25
TCAACGC830150.022.9630912-13
Produced by FastQC (version 0.11.5)