FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200428

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200428
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7982166
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG88790.11123547167523201No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT500600.074.7163541
AGCAGTG565400.066.060252
CAGTGGT737750.050.531144
GCAGTGG761050.049.1295973
AGTGGTA770950.048.4015965
TGGTATC804650.046.2256747
GTGGTAT807700.046.0712976
GGTATCA821650.045.3221138
GTATCAA835800.044.5152639
ACGACGC2650.033.6717645
CAACGCA822150.026.01042412-13
ATCAACG825200.025.92973710-11
ACGCAGA858300.024.9574714-15
AGAGTAC854150.024.84542818-19
GCAGAGT862850.024.76214616-17
TACATGG848900.024.6004322-23
AGTACAT856550.024.58594720-21
TCAACGC817800.022.7918412-13
CATGGGA343750.022.69439524-25
CATGGGG335750.022.55156724-25
Produced by FastQC (version 0.11.5)