FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200433

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200433
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9265764
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGAAG20000.017.9911861
TCAACGG28450.017.9398337
CGGGAAG13700.017.9046973
GAGACTT40500.017.7690731
GGGGTGA10700.017.5790335
GAGACCC27300.017.5335031
CGATGCG17600.017.4434991
GCATGAC27300.017.4061937
CCCGGAA18050.017.3743951
ACGGCCT29450.017.3680210
AACGGCC29500.017.3385839
CGCATGG27500.017.2459321
CGCTGAG15400.017.2138883
ATACCCG24800.017.2093732
TAACGAC21300.017.2030981
GTAGCGC21550.017.1504158
GCGCATG19700.017.1417375
GCACGTG22250.017.1115021
CGTAAGT31600.017.062831
CGGTGTG16250.016.9226884
Produced by FastQC (version 0.11.5)