FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200434

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200434
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9265764
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT582950.074.162561
AGCAGTG658250.065.561782
CAGTGGT865500.049.8870474
GCAGTGG891000.048.59023
AGTGGTA907300.047.592835
GTGGTAT946750.045.606196
TGGTATC945000.045.605957
GGTATCA963300.044.7618648
GTATCAA978400.044.1151859
ACGACGC2900.032.235765
CAACGCA962200.025.84504912-13
ATCAACG965550.025.77784710-11
ACGCAGA1007000.024.73358714-15
AGAGTAC1000200.024.64093418-19
GCAGAGT1011600.024.52291316-17
TACATGG987850.024.4141722-23
AGTACAT1001450.024.40420220-21
CATGGGA407700.022.6555424-25
TCAACGC956800.022.54855512-13
CATGGGG386950.022.47533424-25
Produced by FastQC (version 0.11.5)