FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200436

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200436
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9279994
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT586950.074.6074451
AGCAGTG662900.066.031712
CAGTGGT870700.050.1856584
GCAGTGG897850.048.7953383
AGTGGTA908300.048.2129065
TGGTATC946100.046.2317437
GTGGTAT948950.046.1060756
GGTATCA968950.045.2523548
GTATCAA981350.044.594689
CAACGCA964500.026.07721112-13
ATCAACG968650.026.01409710-11
ACGCAGA1006250.025.03094514-15
AGAGTAC1001100.024.84406918-19
ACGACGC3600.024.7867345
GCAGAGT1014400.024.73624816-17
TACATGG994050.024.63609322-23
AGTACAT1002850.024.61307120-21
CATGGGA404400.023.12725424-25
TCAACGC959600.022.833612-13
CATGGGG397100.022.59982524-25
Produced by FastQC (version 0.11.5)