FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200438

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200438
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9265907
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT594450.073.722181
AGCAGTG668300.065.447732
CAGTGGT864400.050.5124244
GCAGTGG891150.049.164923
AGTGGTA905350.048.3046235
GTGGTAT947100.046.1221666
TGGTATC945900.046.0955667
GGTATCA966650.045.1344158
GTATCAA985200.044.2757239
ACGACGC3650.030.2710915
CAACGCA969350.025.97942712-13
ATCAACG971600.025.93803610-11
ACGCAGA1011100.024.97746714-15
AGAGTAC1004900.024.72422818-19
TACATGG988400.024.65445722-23
GCAGAGT1017950.024.63038416-17
AGTACAT1001300.024.5970320-21
CATGGGA398500.022.9497524-25
CATGGGG393000.022.89777624-25
TCAACGC962900.022.74165212-13
Produced by FastQC (version 0.11.5)