FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200443

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200443
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8416575
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGACTT39900.018.4511341
ACGGCCT28000.018.42430310
GAGACCC24300.017.9785231
AACGGCC28900.017.964729
ACGGAAG15400.017.9358841
CCCGGAA16450.017.9282841
GCACGTG18600.017.9266661
CGGGAAG11600.017.9230143
TCAACGG29100.017.765657
CAAGGCG11200.017.5796938
ATACCCG25800.017.4815162
GGCTAGT18200.017.4071754
AAGGCGA10850.017.335757
AACGATG19000.017.195622
ATGGTTG34700.017.156851
CATACCC35150.016.999821
GCATGAC24650.016.9567627
CCGAACG21750.016.949441
GGGTCTG17300.016.9201931
TAACGAC19250.016.864021
Produced by FastQC (version 0.11.5)