FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200444

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200444
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8416575
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT538900.073.7924351
AGCAGTG600850.066.0968862
CAGTGGT779150.050.911954
GCAGTGG797750.049.7908943
AGTGGTA816100.048.654865
TGGTATC850650.046.537447
GTGGTAT854200.046.397656
GGTATCA868950.045.6261028
GTATCAA878950.045.0836379
CAACGCA867950.026.25096512-13
ATCAACG868600.026.20654510-11
ACGCAGA904850.025.20240214-15
AGAGTAC900300.025.01882218-19
TACATGG888850.024.8764822-23
GCAGAGT913000.024.86616116-17
AGTACAT898550.024.82270820-21
TCAACGC861350.023.06758312-13
CATGGGA364600.022.94683524-25
CATGGGG350300.022.90687624-25
CGCAGAG882950.022.56457316-17
Produced by FastQC (version 0.11.5)