FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200448

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200448
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9808000
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN176440.17989396411092984No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT631100.073.962791
AGCAGTG709450.065.728932
CAGTGGT928300.050.13534
GCAGTGG953000.048.9257353
AGTGGTA970100.048.005015
GTGGTAT1010850.046.000716
TGGTATC1011750.045.934637
GGTATCA1034450.045.027298
GTATCAA1051000.044.3854339
ACGACGC4100.033.157325
CAACGCA1033100.026.1297212-13
ATCAACG1034850.026.0837310-11
ACGCAGA1075500.025.10979314-15
AGAGTAC1066050.024.99068818-19
GCAGAGT1083300.024.82206716-17
TACATGG1054450.024.77407322-23
AGTACAT1070750.024.68073820-21
CATGGGA433500.023.04499624-25
TCAACGC1028300.022.70779412-13
CATGGGG418050.022.671624-25
Produced by FastQC (version 0.11.5)