FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200450

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200450
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9792068
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN202300.20659578752925326No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT613350.074.2349551
AGCAGTG687000.066.124632
CAGTGGT898500.050.3990144
GCAGTGG922250.049.33693
AGTGGTA941500.048.1844255
GTGGTAT982300.046.1563456
TGGTATC982000.046.1293037
GGTATCA1002000.045.219598
GTATCAA1021350.044.397319
CAACGCA999500.026.10705612-13
ATCAACG1004150.025.99634210-11
ACGCAGA1048200.024.94397514-15
AGAGTAC1039800.024.85540818-19
GCAGAGT1056200.024.65035616-17
TACATGG1031100.024.61941722-23
AGTACAT1043250.024.59904520-21
ACGACGC4250.022.9941145
CATGGGG412800.022.88372424-25
TCAACGC994000.022.82256712-13
CATGGGA412650.022.78904324-25
Produced by FastQC (version 0.11.5)