FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200454

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200454
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9774299
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN163460.16723449937432852No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT618800.074.1416551
AGCAGTG700700.065.352132
CAGTGGT916650.049.8916444
GCAGTGG943200.048.5723653
AGTGGTA961000.047.6272135
TGGTATC996850.045.802117
GTGGTAT999400.045.7133646
GGTATCA1018750.044.909968
GTATCAA1037700.044.1028029
CAACGCA1021050.025.91105512-13
ATCAACG1025150.025.8105110-11
ACGCAGA1066450.024.86332314-15
AGAGTAC1057250.024.76862718-19
GCAGAGT1072050.024.61098316-17
TACATGG1045950.024.60083422-23
AGTACAT1063200.024.46953820-21
CATGGGA432350.023.07748424-25
TCAACGC1015750.022.5650512-13
CATGGGG408650.022.51773624-25
CGCAGAG1037600.022.17560416-17
Produced by FastQC (version 0.11.5)