FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200460

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200460
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8187181
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN101210.12362008364051069No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG94560.11549762977024693No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT526550.074.1778951
AGCAGTG593100.065.709252
CAGTGGT774250.050.1884964
GCAGTGG801800.048.685373
AGTGGTA807600.048.1220975
GTGGTAT841750.046.0898556
TGGTATC841700.046.0053947
GGTATCA861250.045.0241628
GTATCAA873700.044.3390739
ACGACGC2950.031.6856675
CAACGCA859850.026.05984312-13
ATCAACG861800.025.98997910-11
ACGCAGA894600.025.0831214-15
AGAGTAC891800.024.80790918-19
GCAGAGT905250.024.75357416-17
AGTACAT889200.024.68717420-21
TACATGG879150.024.66678422-23
CGACGCT3950.023.6644126
CATGGGA354900.022.8733924-25
TCAACGC854600.022.73886112-13
Produced by FastQC (version 0.11.5)