FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200466

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200466
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9460374
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN191960.20290952556421132No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT598250.074.41111
AGCAGTG671350.066.1791152
CAGTGGT883350.050.3338244
GCAGTGG903900.049.269673
AGTGGTA927000.047.960175
TGGTATC967850.045.8607837
GTGGTAT970900.045.76736
GGTATCA987450.045.04238
GTATCAA1005000.044.298739
ACGACGC3950.027.969235
CAACGCA988100.025.84584812-13
ATCAACG991000.025.7929810-11
ACGCAGA1033550.024.75344714-15
AGAGTAC1020900.024.67130318-19
TACATGG1008150.024.5516522-23
GCAGAGT1034550.024.54073516-17
AGTACAT1022700.024.45794520-21
CATGGGA409150.023.08264224-25
TCAACGC982700.022.64694612-13
CATGGGG406200.022.38702824-25
Produced by FastQC (version 0.11.5)