FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200468

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200468
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9528621
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN116420.1221792744196668No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG96750.10153620340236011No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT612400.074.482771
AGCAGTG689150.066.162932
CAGTGGT897250.050.6809964
GCAGTGG925100.049.263143
AGTGGTA940100.048.440265
TGGTATC986000.046.076887
GTGGTAT990500.045.921386
GGTATCA1008650.045.090198
GTATCAA1023300.044.42539
CAACGCA1006100.025.92861612-13
ATCAACG1009050.025.866610-11
ACGACGC3150.025.6277125
ACGCAGA1047650.024.9436614-15
GCAGAGT1054350.024.69023516-17
AGAGTAC1048700.024.65526218-19
TACATGG1029800.024.610522-23
AGTACAT1047550.024.49804320-21
TCAACGC1001700.022.75245912-13
CATGGGA415650.022.736524-25
CATGGGG415100.022.6847124-25
Produced by FastQC (version 0.11.5)