FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200474

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200474
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8579451
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN175630.2047100682782616No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT543600.073.886731
AGCAGTG612300.065.559972
CAGTGGT798550.050.1164784
GCAGTGG816750.049.124593
AGTGGTA832700.048.1654475
TGGTATC871850.045.871777
GTGGTAT874450.045.7618646
GGTATCA890450.045.015658
GTATCAA907300.044.1661079
CAACGCA889150.026.03662312-13
ATCAACG892050.025.93946310-11
ACGCAGA925350.025.01620314-15
AGAGTAC921900.024.7984118-19
TACATGG910350.024.64681822-23
GCAGAGT936600.024.60659616-17
AGTACAT920400.024.60137220-21
CATGGGA367100.022.72213724-25
TCAACGC884550.022.71012912-13
CATGGGG364650.022.70579324-25
CGCAGAG903800.022.28540416-17
Produced by FastQC (version 0.11.5)