FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200475

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200475
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8648462
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGAAG12650.018.6125073
TCAACGG26400.018.2063757
GAGACTT37250.017.8453641
CCCGAAG20450.017.8135071
CCCGGAA17700.017.7831021
ACGGCCT27000.017.76156810
AACGGCC27250.017.7196049
ACGGAAG16050.017.6912861
GCGCATG19150.017.5233735
GGCTAGT18150.017.5187384
ACGCGCT12500.017.5105239
AGAGAGT19750.017.486838
AAGGCGA9650.017.438547
GCCGTGA12500.017.3448661
CGCATGG26800.017.3297561
CGTAAGT29200.017.299941
GGGGTGA8850.017.2805755
ACTATGG31700.017.2548771
GTGGTAG16700.017.1953355
GAGACCC24350.017.1751041
Produced by FastQC (version 0.11.5)