FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200476

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200476
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8648462
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN106920.12362891806658803No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT544150.074.337641
AGCAGTG615550.065.565042
CAGTGGT806300.049.986644
GCAGTGG827000.048.8918953
AGTGGTA847850.047.593215
GTGGTAT885450.045.4426356
TGGTATC885100.045.4200337
GGTATCA902950.044.538598
GTATCAA916300.043.8865749
CAACGCA899850.026.02063412-13
ATCAACG902750.025.92179310-11
ACGCAGA939250.024.94085714-15
AGAGTAC929500.024.78518718-19
GCAGAGT943150.024.62453816-17
TACATGG921050.024.5667122-23
AGTACAT930900.024.52198620-21
CATGGGA370450.022.8367824-25
CATGGGG366000.022.63252624-25
TCAACGC895500.022.59716812-13
CGCAGAG914250.022.13023816-17
Produced by FastQC (version 0.11.5)