FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200478

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200478
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8563288
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN143760.16787944070081492No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT546850.074.5058751
AGCAGTG607150.067.035142
CAGTGGT796600.051.0485534
GCAGTGG821400.049.5842443
AGTGGTA838350.048.5443655
TGGTATC873500.046.4582447
GTGGTAT879350.046.2450186
GGTATCA892950.045.5182278
GTATCAA906700.044.809729
ACGACGC3550.031.1228755
CAACGCA890050.026.00558712-13
ATCAACG893250.025.93315310-11
ACGCAGA931300.024.89066914-15
AGAGTAC924550.024.79419718-19
GCAGAGT936400.024.69851316-17
TACATGG912350.024.57901822-23
AGTACAT924100.024.54447220-21
CATGGGA371350.023.03449824-25
TCAACGC884750.022.94764312-13
CGCAGAG906900.022.47439816-17
Produced by FastQC (version 0.11.5)