FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200480

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200480
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9934215
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN211140.2125381824331364No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC104110.10479942300423335No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT526500.072.117671
AGCAGTG598550.063.2894022
CAGTGGT752750.050.207854
GCAGTGG791300.047.926363
AGTGGTA792050.047.7092825
TGGTATC808700.046.5893977
GTGGTAT810000.046.5072676
GGTATCA822000.045.8976148
GTATCAA841400.044.9451839
CAACGCA819450.026.98105212-13
ATCAACG821600.026.86444510-11
AGAGTAC856400.025.46204218-19
ACGCAGA873800.025.3389914-15
AGTACAT855200.025.24565920-21
TACATGG850000.025.17821722-23
GCAGAGT875550.025.1282916-17
CATGGGA369300.023.30352224-25
TCAACGC814650.023.2934312-13
CGCAGAG833500.022.79771416-17
CATGGGG332600.022.7594424-25
Produced by FastQC (version 0.11.5)