FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200482

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200482
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9766252
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN234160.23976444597169927No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC104760.10726735292105917No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT518350.072.2334441
AGCAGTG578900.064.552732
CAGTGGT732250.050.9069824
GCAGTGG770250.048.5803383
AGTGGTA774300.048.1715435
TGGTATC789700.047.1487167
GTGGTAT794100.046.875326
GGTATCA803150.046.4656878
GTATCAA822450.045.4370739
CAACGCA805000.026.79144712-13
ATCAACG804550.026.76013210-11
AGAGTAC840250.025.29008518-19
ACGCAGA855850.025.22849814-15
AGTACAT835150.025.21306620-21
ACGACGC3550.025.138815
TACATGG826300.025.12120422-23
GCAGAGT855900.025.077316-17
TCAACGC797850.023.48148512-13
CGCAGAG816050.023.00667616-17
CATGGGA356900.022.90267624-25
Produced by FastQC (version 0.11.5)