FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200484

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200484
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9831689
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN149050.1516016220610721No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC104860.10665512304142248No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT509500.072.328391
AGCAGTG576600.063.836542
CAGTGGT729950.050.226974
GCAGTGG763950.048.2411583
AGTGGTA771600.047.5024155
TGGTATC783150.046.7435847
GTGGTAT784800.046.611876
GGTATCA797450.046.0012978
GTATCAA814850.045.0493779
CAACGCA792200.026.96831512-13
ATCAACG792550.026.94382710-11
AGAGTAC824900.025.45384818-19
ACGCAGA846000.025.28660214-15
TACATGG818350.025.14697522-23
AGTACAT827250.025.1247820-21
GCAGAGT846850.025.04509516-17
TCAACGC786100.023.42056512-13
CATGGGA359200.023.35866224-25
CGCAGAG805400.022.88139316-17
TATCAAC820200.022.44753810-11
Produced by FastQC (version 0.11.5)