FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200488

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200488
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9736692
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN205950.2115194770462083No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC104560.10738760145642894No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG97950.1005988481508915No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT519050.072.511731
AGCAGTG581150.064.537772
CAGTGGT735400.050.8345074
GCAGTGG775350.048.4047973
AGTGGTA774400.048.2105525
TGGTATC792100.047.0503467
GTGGTAT796650.046.785756
GGTATCA807650.046.2343838
GTATCAA823400.045.3966879
ACGACGC3050.032.0401735
CAACGCA801900.027.1158712-13
ATCAACG804050.027.03314210-11
AGAGTAC836150.025.71276518-19
AGTACAT836250.025.45692820-21
ACGCAGA855950.025.4506214-15
TACATGG831850.025.39671522-23
GCAGAGT859250.025.22139216-17
TCAACGC798250.023.49520712-13
CATGGGA362200.023.38071324-25
CGCAGAG816150.023.05554616-17
Produced by FastQC (version 0.11.5)