FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200490

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200490
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9558656
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN226230.23667553262718108No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG111540.11669004512768323No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC100640.10528676834902313No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT500250.072.2492451
AGCAGTG563600.064.089762
CAGTGGT722250.049.9992564
GCAGTGG758550.047.7637563
AGTGGTA759400.047.582015
TGGTATC784100.045.9943737
GTGGTAT788300.045.8123326
GGTATCA797550.045.320918
GTATCAA812600.044.4658439
CAACGCA792750.026.46455812-13
ATCAACG794250.026.40321510-11
ACGCAGA840450.024.98438814-15
AGAGTAC828800.024.95134518-19
TACATGG816750.024.80685822-23
AGTACAT827900.024.69109220-21
GCAGAGT846200.024.68579516-17
TCAACGC786050.023.04098512-13
CATGGGA355850.022.68842124-25
CGCAGAG804100.022.56556316-17
TATCAAC818700.022.15234210-11
Produced by FastQC (version 0.11.5)