FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200492

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200492
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9636009
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN147510.1530820488025696No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC103970.10789736705310259No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG96780.10043577169759803No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT504050.072.449421
AGCAGTG569500.064.068472
CAGTGGT720350.050.517574
GCAGTGG758450.048.1288833
AGTGGTA762450.047.8025975
TGGTATC774950.046.8702137
GTGGTAT777450.046.7508286
GGTATCA788650.046.1755148
GTATCAA807250.045.0584649
CAACGCA784950.026.85481812-13
ATCAACG787300.026.75923510-11
ACGACGC3350.025.3695455
AGAGTAC820750.025.36513118-19
ACGCAGA837950.025.22769414-15
AGTACAT821650.025.07975820-21
TACATGG812200.025.06948722-23
GCAGAGT839450.025.01251416-17
TCAACGC780250.023.37842812-13
CATGGGA348900.023.16657324-25
CGCAGAG800000.022.90014516-17
Produced by FastQC (version 0.11.5)