FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200494

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200494
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9617040
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN191750.19938567376240507No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG105980.11020022792875978No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC102250.10632169565687573No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT498350.072.9029161
AGCAGTG565250.064.1530762
CAGTGGT717200.050.4457664
GCAGTGG756950.047.894013
AGTGGTA758650.047.74055
TGGTATC770750.046.8054777
GTGGTAT776400.046.519376
GGTATCA783800.046.135378
GTATCAA799850.045.2733769
CAACGCA780500.026.97637612-13
ATCAACG781500.026.91761210-11
AGAGTAC815500.025.43349618-19
ACGCAGA834400.025.28267714-15
TACATGG805700.025.26027522-23
AGTACAT815450.025.23234220-21
GCAGAGT834850.025.05606816-17
ACGACGC3500.024.2884525
CATGGGA347950.023.57834224-25
TCAACGC775100.023.42195712-13
CGCAGAG792950.022.91646216-17
Produced by FastQC (version 0.11.5)