FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200496

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200496
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11257307
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN242950.21581538106760348No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC119710.10633981999424906No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT593050.072.307041
AGCAGTG660850.064.741362
CAGTGGT844450.050.5296554
GCAGTGG882300.048.5427743
AGTGGTA888200.048.0574265
TGGTATC901950.047.2583667
GTGGTAT906350.047.0170486
GGTATCA920900.046.3846478
GTATCAA941150.045.423159
CAACGCA914800.027.08644112-13
ATCAACG916950.026.99025310-11
AGAGTAC958350.025.5143218-19
ACGCAGA979200.025.33262814-15
AGTACAT957650.025.30906920-21
TACATGG952000.025.2198222-23
GCAGAGT980850.025.12843516-17
CATGGGA406850.023.63791824-25
TCAACGC909750.023.5703512-13
CGCAGAG932450.023.03868716-17
TATCAAC948550.022.63289310-11
Produced by FastQC (version 0.11.5)