FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200498

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200498
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11072716
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN264760.23911025984952564No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG123220.11128254350603772No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC116270.10500585402894826No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT576000.072.185031
AGCAGTG646250.064.2257162
CAGTGGT829400.049.94214
GCAGTGG870450.047.819583
AGTGGTA876450.047.3439455
TGGTATC891400.046.43067
GTGGTAT899450.046.0651476
GGTATCA909400.045.6424458
GTATCAA927050.044.7732629
ACGACGC3800.032.4323355
CAACGCA902350.026.63537412-13
ATCAACG905500.026.50962310-11
AGAGTAC943250.025.18896718-19
ACGCAGA960550.025.0767614-15
TACATGG931650.024.97878622-23
AGTACAT945250.024.87965620-21
GCAGAGT965250.024.80427716-17
CGACGCT5500.023.9545736
TCAACGC897600.023.20646512-13
CATGGGA401600.022.89131424-25
Produced by FastQC (version 0.11.5)