FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200500

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200500
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11136459
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN169440.15214890119022573No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC119100.10694602296834209No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT579950.072.479951
AGCAGTG658450.063.798372
CAGTGGT834700.050.257324
GCAGTGG874050.048.1624953
AGTGGTA874950.047.9954345
TGGTATC893400.046.911037
GTGGTAT898050.046.6582456
GGTATCA909500.046.263678
GTATCAA931150.045.2199559
CAACGCA905150.027.04894812-13
ATCAACG907800.026.95728110-11
AGAGTAC946700.025.49591818-19
ACGCAGA967550.025.3413314-15
TACATGG937650.025.21956322-23
AGTACAT949000.025.19687720-21
GCAGAGT972200.025.01345616-17
TCAACGC899850.023.41851612-13
CATGGGA407500.023.24207724-25
CGCAGAG919700.022.92135416-17
CATGGGG375350.022.54548524-25
Produced by FastQC (version 0.11.5)