FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200506

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200506
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12743919
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN304270.2387570103042871No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC135620.10641938323682064No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT655300.072.006531
AGCAGTG740400.063.5658842
CAGTGGT944600.049.752514
GCAGTGG998750.047.2503663
AGTGGTA1002150.046.9044345
TGGTATC1010750.046.3150447
GTGGTAT1018550.046.0211376
GGTATCA1033450.045.4300358
GTATCAA1057450.044.41529
ACGACGC4800.030.101625
CAACGCA1028850.026.6766312-13
ATCAACG1029650.026.64324210-11
AGAGTAC1076550.025.19731118-19
ACGCAGA1098200.025.03889714-15
AGTACAT1076400.024.9593920-21
TACATGG1066250.024.90039422-23
GCAGAGT1099100.024.85280616-17
TCAACGC1021150.023.08863612-13
CATGGGA473700.023.03865224-25
CGCAGAG1044350.022.6594716-17
Produced by FastQC (version 0.11.5)