FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200510

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200510
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12818462
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN260380.2031288933102895No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC138320.1079068612131471No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT676300.072.237741
AGCAGTG760300.064.167732
CAGTGGT955850.050.91764
GCAGTGG1006400.048.5346073
AGTGGTA1009550.048.2648625
TGGTATC1034150.047.002417
GTGGTAT1036100.046.8811346
GGTATCA1053100.046.270188
GTATCAA1076800.045.303119
CAACGCA1052250.026.89002212-13
ATCAACG1052250.026.87161610-11
AGAGTAC1095050.025.40170318-19
ACGCAGA1121100.025.29538514-15
TACATGG1080200.025.21364822-23
AGTACAT1093750.025.18680420-21
GCAGAGT1120100.025.09640116-17
TCAACGC1045050.023.44355612-13
CATGGGA464750.023.18286124-25
CGCAGAG1068100.022.93918616-17
CATGGGG432500.022.88344224-25
Produced by FastQC (version 0.11.5)