FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200522

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200522
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8155332
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG97880.1200196386854637No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC86180.10567319638244035No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT429250.072.359591
AGCAGTG483400.064.162712
CAGTGGT612800.050.52114
AGTGGTA640150.048.4109155
GCAGTGG644500.048.2432173
TGGTATC660900.046.8470847
GTGGTAT661950.046.7789156
GGTATCA671950.046.1411368
GTATCAA685050.045.272059
ACGACGC2050.035.2720685
CAACGCA667500.027.13219512-13
ATCAACG669000.027.0666710-11
ACGCAGA707250.025.6115614-15
AGAGTAC696450.025.59379418-19
AGTACAT695050.025.41880220-21
TACATGG691450.025.26122522-23
GCAGAGT714900.025.20931616-17
TCAACGC663200.023.4440612-13
CATGGGA303300.023.35018724-25
CGACGCT3150.022.9552636
Produced by FastQC (version 0.11.5)