FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200524

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200524
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8263485
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC90490.10950585618537458No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT444950.072.382761
AGCAGTG498300.064.5274352
CAGTGGT633350.050.6642074
GCAGTGG666600.048.3423083
AGTGGTA665900.048.197585
TGGTATC680350.047.1156967
GTGGTAT684800.046.8196756
GGTATCA691250.046.385628
GTATCAA707800.045.3676649
ACGACGC2600.029.441445
CAACGCA689500.027.03975512-13
ATCAACG689650.027.00842510-11
AGAGTAC718950.025.5478118-19
ACGCAGA731600.025.51266114-15
AGTACAT719500.025.31293320-21
TACATGG715850.025.2408522-23
GCAGAGT736700.025.217216-17
TCAACGC684650.023.50749612-13
CGCAGAG700700.022.991716-17
CATGGGA309600.022.97504824-25
Produced by FastQC (version 0.11.5)