FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200525

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200525
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8252646
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAGAT27950.020.5430989
CTCGGAG28950.020.2133227
CGTGTGC28300.019.3172195
TTGTGAG47400.019.3072156
TCGGAGA29800.019.3045658
GAGTAAT43800.019.06108310
CCGCTAG27450.019.03404610
GCTCGAC22700.018.6071556
CTTGTGA48000.018.4929815
GGCATCC35750.018.3684676
CCGGGTA24400.018.3598351
GACTCTC47150.018.3253421
CTGGACG13400.018.3174171
GGGTAAG27450.018.3125343
CGGGCAC25650.018.1824784
GAATGCT38250.018.0594882
AGGTCGC22750.018.0344146
TTGCGTC27500.018.0112551
CACCGCT29700.017.999258
GCCGATG31700.017.916561
Produced by FastQC (version 0.11.5)