FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200526

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200526
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8252646
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG92860.11252148704791165No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC90120.10920133978847511No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT435350.072.7634051
AGCAGTG493250.064.122942
CAGTGGT631550.050.010224
GCAGTGG656300.048.2114683
AGTGGTA659050.047.9313665
TGGTATC674750.046.736157
GTGGTAT680200.046.4046066
GGTATCA687200.045.9454158
GTATCAA702350.045.0028049
CAACGCA682200.026.82940712-13
ATCAACG684100.026.78833810-11
AGAGTAC709500.025.38889718-19
ACGCAGA726150.025.26885214-15
AGTACAT709100.025.17836220-21
TACATGG706200.025.07280222-23
GCAGAGT729350.024.95830516-17
ACGACGC3800.024.6242965
TCAACGC678350.023.30449512-13
CGCAGAG695400.022.76986516-17
CATGGGA311250.022.75886224-25
Produced by FastQC (version 0.11.5)