FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200528

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200528
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9522757
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC103930.10913856144811843No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT504850.072.960621
AGCAGTG573400.064.1433942
CAGTGGT730200.050.2128224
GCAGTGG759800.048.4637343
AGTGGTA763150.048.0367135
TGGTATC780850.046.8708277
GTGGTAT786750.046.5291676
GGTATCA795750.046.042288
GTATCAA814000.045.0786489
ACGACGC2500.027.2211385
CAACGCA789650.027.04575212-13
ATCAACG792000.027.0079210-11
AGAGTAC826500.025.48449718-19
ACGCAGA839500.025.4827214-15
GCAGAGT842900.025.22390716-17
AGTACAT827950.025.20356220-21
TACATGG826950.024.98689522-23
TCAACGC785650.023.41263412-13
CATGGGA359750.023.35847524-25
CGCAGAG803250.022.86765916-17
Produced by FastQC (version 0.11.5)